by E. Punzón-Quijorna, V. Sánchez-Vaquero, A. Muñoz Noval, D. Gallach Pérez, A. Climent Font, G. Ceccone, R. Gago, J. P. García Ruiz and M. Manso Silván
Abstract:
Deposition of allylamine (ALL) by plasma enhanced chemical vapor deposition has been optimized on silicon based models. Simultaneous energy recoil detection analysis and Rutherford backscattering spectra show that 100 W deposition is ideal in terms of polymerized film formation and H content while, lower or higher power induce reduced film retention or excessive cross linking, respectively. Surface composition of the ALL film was further probed by X-ray photoelectron spectroscopy revealing a monocomponent N 1s spectrum compatible with the presence of primary amines. Optimized ALL films were applied to polycaprolactone (PCL) surfaces after Ar plasma activation with implications in the chemistry and wettability of this biocompatible polymer. Human mesenchymal stem cells (hMSCs) were cultured on ALL coated PCL surface and controls. ALL functionalized PCL was found to be especially attractive for the formation of confluent monolayers of hMSCs after 72 h of culture.
Reference:
E. Punzón-Quijorna, V. Sánchez-Vaquero, A. Muñoz Noval, D. Gallach Pérez, A. Climent Font, G. Ceccone, R. Gago, J. P. García Ruiz and M. Manso Silván, “Optimized allylamine deposition for improved pluripotential cell culture”, Vacuum, vol. 85, no. 12, pp. 1071–1075.
Bibtex Entry:
@article{punzon-quijorna_optimized_2011, series = {Selected {Papers} from the 11th {European} {Vacuum} {Conference}, {EVC}-11 8th {Iberian} {Vacuum} {Meeting}, {IVM}-8 6th {European} {Topical} {Conference} on {Hard} {Coatings}, {ETCHC}-6, 20-24 {September} 2010, {Salamanca}, {Spain}}, title = {Optimized allylamine deposition for improved pluripotential cell culture}, volume = {85}, issn = {0042-207X}, url = {http://www.sciencedirect.com/science/article/pii/S0042207X11000765}, doi = {10.1016/j.vacuum.2010.12.017}, abstract = {Deposition of allylamine (ALL) by plasma enhanced chemical vapor deposition has been optimized on silicon based models. Simultaneous energy recoil detection analysis and Rutherford backscattering spectra show that 100 W deposition is ideal in terms of polymerized film formation and H content while, lower or higher power induce reduced film retention or excessive cross linking, respectively. Surface composition of the ALL film was further probed by X-ray photoelectron spectroscopy revealing a monocomponent N 1s spectrum compatible with the presence of primary amines. Optimized ALL films were applied to polycaprolactone (PCL) surfaces after Ar plasma activation with implications in the chemistry and wettability of this biocompatible polymer. Human mesenchymal stem cells (hMSCs) were cultured on ALL coated PCL surface and controls. ALL functionalized PCL was found to be especially attractive for the formation of confluent monolayers of hMSCs after 72 h of culture.}, number = {12}, urldate = {2017-10-09}, journal = {Vacuum}, author = {Punzón-Quijorna, E. and Sánchez-Vaquero, V. and Muñoz Noval, A. and Gallach Pérez, D. and Climent Font, A. and Ceccone, G. and Gago, R. and García Ruiz, J. P. and Manso Silván, M.}, month = jun, year = {2011}, keywords = {PE-CVD, RBS, ERDA, XPS, Allylamine, Polycaprolactone}, pages = {1071--1075}, file = {ScienceDirect Full Text PDF:E:\cmam_papers\files\1129\Punzón-Quijorna et al. - 2011 - Optimized allylamine deposition for improved pluri.pdf:application/pdf;ScienceDirect Full Text PDF:E:\Usuarios\Administrator\Zotero\storage\EFJY64LP\Punzón-Quijorna et al. - 2011 - Optimized allylamine deposition for improved pluri.pdf:application/pdf;ScienceDirect Snapshot:E:\cmam_papers\files\1123\S0042207X11000765.html:text/html;ScienceDirect Snapshot:E:\Usuarios\Administrator\Zotero\storage\5ZPL9UI9\S0042207X11000765.html:text/html}, }