by Esther Punzón-Quijorna, Vanessa Sánchez-Vaquero, Álvaro Muñoz-Noval, M. Jesus Pérez-Roldán, Raúl J. Martín-Palma, Francois Rossi, Aurelio Climent-Font, Miguel Manso-Silván, J Predestinacion García Ruiz and Vicente Torres-Costa
Abstract:
The localized irradiation of Si allows a precise patterning at the microscale of nanostructured materials such as porous silicon (PS). PS patterns with precisely defined geometries can be fabricated using ion stopping masks. The nanoscale textured micropatterns were used to explore their influence as microenvironments for human mesenchymal stem cells (hMSCs). In fact, the change of photoluminescence emission from PS upon aging in physiological solution suggests the intense formation of silanol surface groups, which may play a relevant role in ulterior cell adhesion. The experimental results show that hMSCs are sensitive to the surface micropatterns. In this regard, preliminary β-catenin labeling studies reveal the formation of cell to cell interaction structures, while microtubule orientation is strongly influenced by the selective adhesion conditions. Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of HMSCs.
Reference:
Esther Punzón-Quijorna, Vanessa Sánchez-Vaquero, Álvaro Muñoz-Noval, M. Jesus Pérez-Roldán, Raúl J. Martín-Palma, Francois Rossi, Aurelio Climent-Font, Miguel Manso-Silván, J Predestinacion García Ruiz and Vicente Torres-Costa, “Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research”, Nanoscale Research Letters, vol. 7, pp. 396.
Bibtex Entry:
@article{punzon-quijorna_nanostructured_2012,
title = {Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research},
volume = {7},
issn = {1556-276X},
url = {https://doi.org/10.1186/1556-276X-7-396},
doi = {10.1186/1556-276X-7-396},
abstract = {The localized irradiation of Si allows a precise patterning at the microscale of nanostructured materials such as porous silicon (PS). PS patterns with precisely defined geometries can be fabricated using ion stopping masks. The nanoscale textured micropatterns were used to explore their influence as microenvironments for human mesenchymal stem cells (hMSCs). In fact, the change of photoluminescence emission from PS upon aging in physiological solution suggests the intense formation of silanol surface groups, which may play a relevant role in ulterior cell adhesion. The experimental results show that hMSCs are sensitive to the surface micropatterns. In this regard, preliminary β-catenin labeling studies reveal the formation of cell to cell interaction structures, while microtubule orientation is strongly influenced by the selective adhesion conditions. Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of HMSCs.},
urldate = {2017-10-10},
journal = {Nanoscale Research Letters},
author = {Punzón-Quijorna, Esther and Sánchez-Vaquero, Vanessa and Muñoz-Noval, Álvaro and Pérez-Roldán, M. Jesus and Martín-Palma, Raúl J. and Rossi, Francois and Climent-Font, Aurelio and Manso-Silván, Miguel and Ruiz, J Predestinacion García and Torres-Costa, Vicente},
month = jul,
year = {2012},
keywords = {Cell adhesion, Cell guide, Human mesenchymal stem cells, Ion beam, Micropattern, Nanostructured porous silicon, Photoluminescence},
pages = {396},
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}