by G. Pérez Liñero
Abstract:
Proton therapy presents a promising alternative to conventional radiation therapy in the treatment of malignant tumors due to better dose distribution that can spare healthy tissue without sacrificing dose delivery to the tumor volume. In this work, we study the effect of different proton beam energies on glioblastoma cells, also known as glioblastoma multiforme (GBM), a highly aggressive and invasive form of brain cancer with a poor prognosis.. We also compare the experimental results with computer simulations. Glioblastoma cells (U-87 MG) were irradiated with energies ranging from 1.5 to 8.5 MeV with a dose of 3.5 Gy. Multiple assays were performed post-irradiation on glioblastoma cells to quantify cell damage and viability by Alamar blue assay, cell cycle by flow cytometry and the generation of reactive oxygen species (ROS). TOPAS software was used to simulate and determine the dose delivered to the cells, as well as other relevant quantities such as cell survival fraction and radiobiological effectiveness. Our viability assay results show a decrease in cell survival after proton irradiation. No significant differences were observed in cell cycle arrest distribution across different proton energies after flow cytometry analysis. We observed variability in ROS production 3 days post-irradiation. Additionally, simulations with TOPAS demonstrated that the Bragg peak lands within the cell monolayer when irradiated at 1.48 MeV.
Reference:
G. Pérez Liñero, “Exploring the theoretical and experimental frontiers of proton therapy”, Graduation Work, Universidad Autónoma de Madrid, Madrid, Spain, Supervisor: Célia Tavares de Sousa Co-supervisor: Belén Cortés Llanos.
Bibtex Entry:
@phdthesis{perez_linero_exploring_2024,
address = {Madrid, Spain},
type = {Graduation {Work}},
title = {Exploring the theoretical and experimental frontiers of proton therapy},
abstract = {Proton therapy presents a promising alternative to conventional radiation therapy in the
treatment of malignant tumors due to better dose distribution that can spare healthy tissue
without sacrificing dose delivery to the tumor volume. In this work, we study the effect
of different proton beam energies on glioblastoma cells, also known as glioblastoma
multiforme (GBM), a highly aggressive and invasive form of brain cancer with a poor
prognosis.. We also compare the experimental results with computer simulations.
Glioblastoma cells (U-87 MG) were irradiated with energies ranging from 1.5 to 8.5
MeV with a dose of 3.5 Gy. Multiple assays were performed post-irradiation on
glioblastoma cells to quantify cell damage and viability by Alamar blue assay, cell cycle
by flow cytometry and the generation of reactive oxygen species (ROS). TOPAS software
was used to simulate and determine the dose delivered to the cells, as well as other
relevant quantities such as cell survival fraction and radiobiological effectiveness.
Our viability assay results show a decrease in cell survival after proton irradiation. No
significant differences were observed in cell cycle arrest distribution across different
proton energies after flow cytometry analysis. We observed variability in ROS production
3 days post-irradiation. Additionally, simulations with TOPAS demonstrated that the
Bragg peak lands within the cell monolayer when irradiated at 1.48 MeV.},
school = {Universidad Autónoma de Madrid},
author = {Pérez Liñero, G.},
year = {2024},
note = {Supervisor: Célia Tavares de Sousa Co-supervisor: Belén Cortés Llanos},
file = {Pérez Liñero - Exploring the theoretical and experimental frontie.pdf:E:\Usuarios\Administrator\Zotero\storage\N7MPI7WD\Pérez Liñero - Exploring the theoretical and experimental frontie.pdf:application/pdf},
}