by M. Manso-Silván, C. Rodríguez-Navas, M. Arroyo-Hernández, E. López-Elvira, R. Gago, L. Vázquez, F. Agulló-Rueda, A. Climent, J. M. Martínez-Duart and J. P. García-Ruiz
Abstract:
The properties of hybrid aminopropyltriethoxysilane–tetraisopropylorthotitanate (APTS-TIPT) platforms prepared by a sol–gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface-oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino-silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet–visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH-dependent surface charge-induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin-coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS-TIPT stimulated process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007
Reference:
M. Manso-Silván, C. Rodríguez-Navas, M. Arroyo-Hernández, E. López-Elvira, R. Gago, L. Vázquez, F. Agulló-Rueda, A. Climent, J. M. Martínez-Duart and J. P. García-Ruiz, “Hybrid titania–aminosilane platforms evaluated with human mesenchymal stem cells”, Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 83B, no. 1, pp. 232–239.
Bibtex Entry:
@article{manso-silvan_hybrid_2007,
title = {Hybrid titania–aminosilane platforms evaluated with human mesenchymal stem cells},
volume = {83B},
issn = {1552-4981},
url = {http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.30788/abstract},
doi = {10.1002/jbm.b.30788},
abstract = {The properties of hybrid aminopropyltriethoxysilane–tetraisopropylorthotitanate (APTS-TIPT) platforms prepared by a sol–gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface-oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino-silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet–visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH-dependent surface charge-induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin-coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS-TIPT stimulated process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007},
language = {en},
number = {1},
urldate = {2017-07-21},
journal = {Journal of Biomedical Materials Research Part B: Applied Biomaterials},
author = {Manso-Silván, M. and Rodríguez-Navas, C. and Arroyo-Hernández, M. and López-Elvira, E. and Gago, R. and Vázquez, L. and Agulló-Rueda, F. and Climent, A. and Martínez-Duart, J. M. and García-Ruiz, J. P.},
month = oct,
year = {2007},
keywords = {aminosilane, hybrid materials, mesenchymal stem cell, sol–gel},
pages = {232--239},
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}