by U. Cuevas Gómez
Abstract:
Proton therapy is a newer and more advanced radiation treatment for tumors that uses energy from positively charged particles (protons). Recent studies suggested that proton therapy is a promising radiation treatment because it focuses more energy on the tumor, causing fewer side effects than traditional radiation. In this work, we study the effect of different proton beam energies and doses on glioblastoma cells responsible for glioblastoma multiforme (GBM), an aggressive and highly invasive malignant brain tumor with a poor prognosis. The irradiation experiments were carried out in the implementation beamline at the CMAM accelerator. Glioblastoma cells, U87MG cell line, were irradiated with energies of 3.65 and 8.47 MeV and doses ranging from 1.3 to 23.3 Gy. Multiple post-irradiation assays were performed on U87MG cells to quantify cell viability by Alamar Blue assay, indentify cell damage by analysing the cell cycle with flow cytometry and cell migration by optical microscopy. Viability assays showed no relation between the energy or dose applied and cell viability, but it did decrease with time up to less than 50% 72 h after irradiation. For the cells irradiated with 8.47 MeV, we observed how the cell cycle changed with the dose applied, a tendency that did not appear for those cells irradiated with 3.65 MeV. Additionally, the migration assay revealed that higher-dose irradiations improved the migration capacity of U87MG cells. Finally, in order to establish if the irradiation occurred at the Bragg peak via simulations with the TOPAS software, it was necessary to determine the thickness of the monolayer of cells. For that purpose, we took images of untreated cells with Atomic Force Microscopy (AFM) and studied their topography, obtaining the mean thickness of an attached cell and the monolayer as a whole.
Reference:
U. Cuevas Gómez, “Exploring the limits between proton therapy and biological systems”, Graduation Work, Universidad Autónoma de Madrid, Madrid, Spain, Supervisor: Célia Tavares de Sousa Co-supervisor: Belén Cortés Llanos.
Bibtex Entry:
@phdthesis{cuevas_gomez_exploring_nodate,
address = {Madrid, Spain},
type = {Graduation {Work}},
title = {Exploring the limits between proton therapy and biological systems},
abstract = {Proton therapy is a newer and more advanced radiation treatment for tumors that
uses energy from positively charged particles (protons). Recent studies suggested that
proton therapy is a promising radiation treatment because it focuses more energy
on the tumor, causing fewer side effects than traditional radiation. In this work, we
study the effect of different proton beam energies and doses on glioblastoma cells
responsible for glioblastoma multiforme (GBM), an aggressive and highly invasive
malignant brain tumor with a poor prognosis. The irradiation experiments were
carried out in the implementation beamline at the CMAM accelerator. Glioblastoma
cells, U87MG cell line, were irradiated with energies of 3.65 and 8.47 MeV and doses
ranging from 1.3 to 23.3 Gy. Multiple post-irradiation assays were performed on
U87MG cells to quantify cell viability by Alamar Blue assay, indentify cell damage
by analysing the cell cycle with flow cytometry and cell migration by optical microscopy.
Viability assays showed no relation between the energy or dose applied and cell
viability, but it did decrease with time up to less than 50% 72 h after irradiation.
For the cells irradiated with 8.47 MeV, we observed how the cell cycle changed with
the dose applied, a tendency that did not appear for those cells irradiated with 3.65 MeV.
Additionally, the migration assay revealed that higher-dose irradiations improved
the migration capacity of U87MG cells. Finally, in order to establish if the irradiation
occurred at the Bragg peak via simulations with the TOPAS software, it was necessary
to determine the thickness of the monolayer of cells. For that purpose, we took images
of untreated cells with Atomic Force Microscopy (AFM) and studied their topography,
obtaining the mean thickness of an attached cell and the monolayer as a whole.},
school = {Universidad Autónoma de Madrid},
author = {Cuevas Gómez, U.},
note = {Supervisor: Célia Tavares de Sousa Co-supervisor: Belén Cortés Llanos},
}