by Y. C. Xiang, R. M. Martínez-Martínez, V. Torres-Costa, F. Agulló-Rueda, J. P. García-Ruiz and M. Manso-Silván
Abstract:
The formation of hierarchical structures consisting of microstripe barriers decorated with nanorough ablated materials prepared by direct laser writing is described. Linear features of circa 25μm width and 12μm height are achieved on amorphous and crystalline titania and graphitic carbon films deposited on silicon. Ablated protrusions build up barriers decorated by nanoscale Si-film reconstructions, as indicated by EDX maps and micro-Raman spectroscopy. Wettability tests show a dramatic change in water contact angle, which leads to almost full wetting after irradiation, irrespective of the original film composition. Fluorescence microscopy images of human mesenchymal stem cells cultured on 1D and 2D structures demonstrate the short term biocompatibility of the ablated surfaces. It is shown that cells adhere, extend and polarize on feature edges, independently of the type of surface, thus suggesting that the created nanoroughness is at the origin of the antifouling behavior. In particular, irradiated anatase and graphite surfaces demonstrate an increased performance of crystalline films for the creation of cell guiding and trapping devices. The results suggest that such laser processing of films may serve as a time-and-cost-efficient method for the design of few-cells analytical surfaces.
Reference:
Y. C. Xiang, R. M. Martínez-Martínez, V. Torres-Costa, F. Agulló-Rueda, J. P. García-Ruiz and M. Manso-Silván, “Direct laser writing of nanorough cell microbarriers on anatase/Si and graphite/Si”, Materials Science and Engineering: C, vol. 66, no. Supplement C, pp. 8–15.
Bibtex Entry:
@article{xiang_direct_2016,
title = {Direct laser writing of nanorough cell microbarriers on anatase/{Si} and graphite/{Si}},
volume = {66},
issn = {0928-4931},
url = {http://www.sciencedirect.com/science/article/pii/S0928493116303575},
doi = {10.1016/j.msec.2016.04.059},
abstract = {The formation of hierarchical structures consisting of microstripe barriers decorated with nanorough ablated materials prepared by direct laser writing is described. Linear features of circa 25μm width and 12μm height are achieved on amorphous and crystalline titania and graphitic carbon films deposited on silicon. Ablated protrusions build up barriers decorated by nanoscale Si-film reconstructions, as indicated by EDX maps and micro-Raman spectroscopy. Wettability tests show a dramatic change in water contact angle, which leads to almost full wetting after irradiation, irrespective of the original film composition. Fluorescence microscopy images of human mesenchymal stem cells cultured on 1D and 2D structures demonstrate the short term biocompatibility of the ablated surfaces. It is shown that cells adhere, extend and polarize on feature edges, independently of the type of surface, thus suggesting that the created nanoroughness is at the origin of the antifouling behavior. In particular, irradiated anatase and graphite surfaces demonstrate an increased performance of crystalline films for the creation of cell guiding and trapping devices. The results suggest that such laser processing of films may serve as a time-and-cost-efficient method for the design of few-cells analytical surfaces.},
number = {Supplement C},
urldate = {2017-11-03},
journal = {Materials Science and Engineering: C},
author = {Xiang, Y. C. and Martínez-Martínez, R. M. and Torres-Costa, V. and Agulló-Rueda, F. and García-Ruiz, J. P. and Manso-Silván, M.},
month = sep,
year = {2016},
keywords = {hMSCs, Cell guides, Cell polarization, Cell traps, Direct laser writing, Focal adhesions, Hierarchical microstructures, Microbarriers, Nanoroughness, Super-hydrophilic transition},
pages = {8--15},
file = {ScienceDirect Full Text PDF:E:\cmam_papers\files\1537\Xiang et al. - 2016 - Direct laser writing of nanorough cell microbarrie.pdf:application/pdf;ScienceDirect Full Text PDF:E:\Usuarios\Administrator\Zotero\storage\MZEFFTHY\Xiang et al. - 2016 - Direct laser writing of nanorough cell microbarrie.pdf:application/pdf;ScienceDirect Snapshot:E:\cmam_papers\files\1532\S0928493116303575.html:text/html;ScienceDirect Snapshot:E:\Usuarios\Administrator\Zotero\storage\89EQ3MGY\S0928493116303575.html:text/html},
}