by Ruth Pulido, Nelson Naveas, Francisco Javier Fernández-Alonso, Miguel Manso-Silván, Leonardo Soriano, Carlos Torres-Ulloa, Karel Mena-Ulecia, Gonzalo Recio-Sánchez, Juan Paulo Garcia-Sandoval and Jacobo Hernández-Montelongo
Abstract:
Urinary catheters (UCs) are critical in biomedical applications, but prolonged use increases the risk of catheter-associated urinary tract infections (CAUTIs), a leading cause of healthcare-associated infections (HAIs). The present study presents a dual strategy to create an antibacterial surface on commercial Foley silicone UCs by combining a contact-killing effect with the controlled release of antimicrobial compounds. We designed a drug delivery system using a layer-by-layer (LbL) antibacterial coating of carboxymethylcellulose (CMC) and chitosan-silver (CHI-Ag) complexes, with ciprofloxacin (CFX) as the model drug. The resulting LbL coating, about 1 μm thick, incorporated Ag0 and demonstrated a high capacity for CFX loading, releasing over twice the amount (70 μg/cm2) compared to uncoated UCs (30 μg/cm2). The antibacterial efficacy was significantly higher in the LbL-coated samples, particularly against S. aureus compared to E. coli. Drug release experiments, modeled using Fick’s second law, indicated a diffusivity of 1.744×10−5 cm2/h. Our mathematical model predicts how variations in drug loading and rest times impact release profiles. Finally, molecular dynamics simulations suggested strong compatibility between CFX and the LbL layers, though with relatively low stability. This dual strategy holds promise for reducing CAUTIs effectively.
Reference:
Ruth Pulido, Nelson Naveas, Francisco Javier Fernández-Alonso, Miguel Manso-Silván, Leonardo Soriano, Carlos Torres-Ulloa, Karel Mena-Ulecia, Gonzalo Recio-Sánchez, Juan Paulo Garcia-Sandoval and Jacobo Hernández-Montelongo, “Drug delivery system based on an antibacterial layer-by-layer coating on urinary catheters: an experimental and simulation approach”, Frontiers in Bioengineering and Biotechnology, vol. 13.
Bibtex Entry:
@article{pulido_drug_2025,
title = {Drug delivery system based on an antibacterial layer-by-layer coating on urinary catheters: an experimental and simulation approach},
volume = {13},
issn = {2296-4185},
shorttitle = {Drug delivery system based on an antibacterial layer-by-layer coating on urinary catheters},
url = {https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1614509/full},
doi = {10.3389/fbioe.2025.1614509},
abstract = {Urinary catheters (UCs) are critical in biomedical applications, but prolonged use increases the risk of catheter-associated urinary tract infections (CAUTIs), a leading cause of healthcare-associated infections (HAIs). The present study presents a dual strategy to create an antibacterial surface on commercial Foley silicone UCs by combining a contact-killing effect with the controlled release of antimicrobial compounds. We designed a drug delivery system using a layer-by-layer (LbL) antibacterial coating of carboxymethylcellulose (CMC) and chitosan-silver (CHI-Ag) complexes, with ciprofloxacin (CFX) as the model drug. The resulting LbL coating, about 1 μm thick, incorporated Ag0 and demonstrated a high capacity for CFX loading, releasing over twice the amount (70 μg/cm2) compared to uncoated UCs (30 μg/cm2). The antibacterial efficacy was significantly higher in the LbL-coated samples, particularly against S. aureus compared to E. coli. Drug release experiments, modeled using Fick’s second law, indicated a diffusivity of 1.744×10−5 cm2/h. Our mathematical model predicts how variations in drug loading and rest times impact release profiles. Finally, molecular dynamics simulations suggested strong compatibility between CFX and the LbL layers, though with relatively low stability. This dual strategy holds promise for reducing CAUTIs effectively.},
language = {English},
urldate = {2025-11-18},
journal = {Frontiers in Bioengineering and Biotechnology},
author = {Pulido, Ruth and Naveas, Nelson and Fernández-Alonso, Francisco Javier and Manso-Silván, Miguel and Soriano, Leonardo and Torres-Ulloa, Carlos and Mena-Ulecia, Karel and Recio-Sánchez, Gonzalo and Garcia-Sandoval, Juan Paulo and Hernández-Montelongo, Jacobo},
month = aug,
year = {2025},
keywords = {drug delivery, molecular dynamics, antibacterial coating, layer-by-layer (LbL), mathematical model, urinary catheters},
file = {Full Text PDF:E:\Usuarios\Administrator\Zotero\storage\P4URKVXA\Pulido et al. - 2025 - Drug delivery system based on an antibacterial layer-by-layer coating on urinary catheters an exper.pdf:application/pdf},
}